English
norskBlar i forfatter "Helland, Ronny"
Viser treff 1-11 av 11
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CorA is a copper repressible surface-associated copper(I)-binding protein produced in Methylomicrobium album BG8
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
The crystal structure of haemoglobin from Atlantic cod
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-16)The crystal structure of haemoglobin from Atlantic cod has been solved to 2.54 A˚ resolution. The structure consists of two tetramers in the crystallographic asymmetric unit. The structure of haemoglobin obtained from one individual cod suggests polymorphism in the tetrameric assembly. -
Discovery of Novel Inhibitor Scaffolds against the Metallo-beta-lactamase VIM-2 by Surface Plasmon Resonance (SPR) Based Fragment Screening
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-10-17)Metallo-β-lactamase (MBL) inhibitors can restore the function of carbapenem antibiotics and therefore help to treat infections of antibiotic resistant bacteria. In this study, we report novel fragments inhibiting the clinically relevant MBL Verona integron-encoded metallo-β-lactamase (VIM-2). The fragments were identified from a library of 490 fragments using an orthogonal screening approach based ... -
Features and structure of a cold active N-acetylneuraminic acid lyase
(Journal article; Peer reviewed, 2019-06-11)N-acetylneuraminate lyases (NALs) are enzymes that catalyze the reversible cleavage and synthesis of sialic acids. They are therefore commonly used for the production of these high-value sugars. This study presents the recombinant production, together with biochemical and structural data, of the NAL from the psychrophilic bacterium Aliivibrio salmonicida LFI1238 (AsNAL). Our characterization shows ... -
The high catalytic rate of the cold-active Vibrio alkaline phosphatase requires a hydrogen bonding network involving a large interface loop
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-16)The role of surface loops in mediating communication through residue networks is still a relatively poorly understood part in the study of cold adaptation of enzymes, especially in terms of their quaternary interactions. Alkaline phosphatase (AP) from the psychrophilic marine bacterium <i>Vibrio splendidus</i> (VAP) is characterized by an analogous large surface loop in each monomer, referred to as ... -
The Methylococcus capsulatus (Bath) secreted protein, MopE*, binds both reduced and oxidized copper.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2012)Under copper limiting growth conditions the methanotrophic bacterium Methylococcus capsulatus (Bath) secrets essentially only one protein, MopE*, to the medium. MopE* is a copper-binding protein whose structure has been determined by X-ray crystallography. The structure of MopE* revealed a unique high affinity copper binding site consisting of two histidine imidazoles and one kynurenine, the latter ... -
Multiple specialised goose-type lysozymes potentially compensate for an exceptional lack of chickentype lysozymes in Atlantic cod
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-06-21)Previous analyses of the Atlantic cod genome showed unique combinations of lacking and expanded number of genes for the immune system. The present study examined lysozyme activity, lysozyme gene distribution and expression in cod. Enzymatic assays employing specific bacterial lysozyme inhibitors provided evidence for presence of g-Type, but unexpectedly not for c-Type lysozyme activity. Database ... -
Psoriasis pathogenesis – Pso p27 constitutes a compact structure forming large aggregates
(Journal article; Tidsskriftartikkel, 2015-07)Psoriasis is a chronic inflammatory skin disease. The absence of microbial organisms as potential causal agents has given rise to the hypothesis that the inflammation is due to an autoimmune reaction. The defined inflamed areas of the skin lesions argue for an immunological disease with a local production of a causal antigen. Pso p27 is a protein generated in mast cells in psoriatic plaques, but ... -
Structural insight into a CE15 esterase from the marine bacterial metagenome
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-12-08)The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal structure of MZ0003, which reveals that residues comprising the catalytic triad differ from previously-characterized fungal homologs, and resolves three large loop regions that are unique to this bacterial ... -
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-13)Chitin is one of the most abundant renewable organic materials found on earth. The chitin utilization locus in <i>Flavobacterium johnsoniae</i>, which encodes necessary proteins for complete enzymatic depolymerization of crystalline chitin, has recently been characterized but no detailed structural information on the enzymes was provided. Here we present protein structures of the <i>F. johnsoniae</i> ... -
X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-15)<i>Background</i> - <i>Para</i>-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs.<br><br> <i>Methods</i> - Cyclohexylamine inhibited four different APs. Co-crystallization with the cold-active <i>Vibrio</i> AP (VAP) was performed and the structure solved.<br><br> ...
